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GeneProof Bordetella pertussis/parapertussis PCR Kit
The kit is designed for the detection of Bordetella pertussis and Bordetella parapertussis genomic DNA based on amplifying specific IS1001 and IS1002 insertion sequences by means of the Polymerase Chain Reaction (PCR) method. Both insertion sequences appear in the B. pertussis and B. parapertussis genome in up to several dozens of copies. Detection is performed by measuring the amplification product concentration growth in the course of the PCR using fluorescence-marked probes (real-time PCR). B. pertussis DNA presence in the sample is indicated by the FAM fluorophore fluorescence growth. B. parapertussis DNA presence in the sample is indicated by the Cy5 fluorophore fluorescence growth. An Internal Standard (IS) is included in the reaction mix, controlling the possible inhibition of the PCR reaction and the efficiency of the DNA isolation process. IS positive amplification is detected in the fluorescence channel for the HEX fluorophore. The detection kit takes an advantage of the “hot start” technology, minimizing non-specific reactions and assuring maximum sensitivity. It contains uracil-DNA-glycosylase (UDG), eliminating possible contamination of the PCR reaction by amplification products. This assures very high sensitivity of the laboratory Bordetella detection in clinical material. The kit is designed for in vitro diagnostics and provides qualitative detection.

Available package sizes: 25, 50, 100 reactions
Platforms: Real-time PCR
Versions: ISIN / ISEX

Real-time PCR kity
BP/ISIN/025 A kit for 25 reactions
BP/ISIN/050 A kit for 50 reactions
BP/ISIN/100 A kit for 100 reactions
BP/ISEX/025 A kit for 25 reactions
BP/ISEX/050 A kit for 50 reactions
BP/ISEX/100 A kit for 100 reactions