The kit is designed for the detection of Hepatitis C virus by polymerase chain reaction. The HCV detection is based on the amplification of a single-copy 5´ UTR sequence by means of the Reverse Transcription Polymerase Chain Reaction (RT-PCR) and on measuring the amplification product concentration in the course of the PCR process by means of a fluorescence marked probe. HCV presence is indicated by FAM fluorophore fluorescence growth. An Internal Standard (IS) is included in the reaction mix, controlling the possible inhibition of the PCR reaction and also the efficiency of the DNA isolation process. IS positive amplification is detected in the JOE fluorophore fluorescence channel. The detection kit takes advantage of the “hot start” technology, minimizing non-specific reactions and assuring maximum sensitivity. Diagnostic kit sensitivity runs in single HCV genome copies per PCR reaction. This assures very high sensitivity of the laboratory detection in clinical material (serum, plasma). The kit is designed for in vitro diagnostics and provides qualitative or quantitative detection.

Available package sizes:	25, 50, 100 reactions
Platforms:	Real-time PCR
Versions:	ISEX

Real-time PCR kity
HCV/ISEX/025	A kit for 25 reactions
HCV/ISEX/050	A kit for 50 reactions
HCV/ISEX/100	A kit for 100 reactions

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