This PCR kit is designed for the detection of Mycoplasma pneumoniae by the
Polymerase Chain Reaction method (PCR). The detection is based on the
principle of amplifying the DNA sequence, specific for M. pneumoniae, of the
M181 gene encoding the CARDS toxin and measuring the amplification
product concentration increase in the course of the PCR by a fluorescencemarked probe (real-time PCR). Mycoplasma presence is indicated by the FAM fluorophore fluorescence growth. An Internal Standard (IS) is included in the reaction mix, controlling the possible inhibition of the PCR reaction and the efficiency of the DNA isolation process. IS positive amplification is detected in the fluorescence channel for the JOE fluorophore. The detection kit takes an advantage of the “hot start” technology, minimizing non-specific reactions and assuring maximum sensitivity. It contains uracil-DNAglycosylase (UDG), eliminating possible contamination of the PCR reaction by amplification products. This diagnostic kit assures very high sensitivity of the laboratory Mycoplasma detection in clinical material. The kit is designed for in vitro diagnostics and provides qualitative detection.
| Available package sizes: | 25, 50, 100 reactions |
| Platforms: | Real-time PCR |
| Versions: | ISIN / ISEX |
| Real-time PCR kits | |
| MP/ISIN/025 | A kit for 25 reactions |
| MP/ISIN/050 | A kit for 50 reactions |
| MP/ISIN/100 | A kit for 100 reactions |
| MP/ISEX/025 | A kit for 25 reactions |
| MP/ISEX/050 | A kit for 50 reactions |
| MP/ISEX/100 | A kit for 100 reactions |
+420 543 211 679

