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GeneProof Mycoplasma species PCR kit
reg. USKVBL ČR
The kit is designed for the detection of the genomic DNA in Mycoplasma species by the Polymerase Chain Reaction method (PCR). This detection is focused on a multi-copy sequence of the gene encoding bacterial 16S RNA, specific for Mycoplasma species. Sensitivity of the PCR detection kit runs in single copies of the Mycoplasma genomic DNA in a reaction. It provides for a sensitive, universal detection of all clinically significant human mycoplasmas and ureaplasmas, including M. pneumoniae, M. hominis, M. genitalium, U. urealitica and M. fermentans. The kit provides for the detection of ones to tens of mycoplasmas in 1 ml of cell-free body fluids (urine, liquor, bronchoalveolar lavage, sputum) or of up to hundreds of mycoplasmas in 1 ml of blood or sperm. The kit is designed and certified for in vitro diagnostics and provides qualitative detection.
The kit is designed also for a sensitive detection of all Mycoplasma strains contaminating tissue cultures and solutions for cell cultures (e.g. virus cultures, BSA) and for the control of contamination in products prepared from cell cultures (e.g. vaccines). It provides for a laboratory detection of ones to tens of mycoplasmas in 1 ml of TK or BSA solution. This method is uniquely adjusted for reaction inhibition detection and due to the simplicity of the whole detection process it provides standard results even during the analysis of materials with high PCR inhibitor contents. This method is able to sensitively detect all known Mycoplasma strains (National Center for Biotechnology Information U.S.; 2006) and it is adjusted to detect at least the following strains of industrially or veterinary significant Mycoplasma species: Acholeplasma axanthum, Mycoplasma alvi, M. arginini, M. buccale, M. cavipharyngis, M. cloacale, M. fastidiosum, M. fermentans, M. genitalium, M. hominis, M. salivarium, M. pulmonis, M. orale, M. penetrans, M.pirum, M. pneumoniae, Ureaplasma urealyticum, U. diversum, U. parvum, Acholeplasma sp., etc.
The product is registered and approved by the State Inspection for Veterinary Bioproducts and Pharmaceuticals, Czech Republic. USKVBL.

Available package sizes: 25, 50, 100 reactions
Platforms: End-point PCR

PCR Kits for "end-point" electrophoretic detection
MSP/E/025 A kit for 25 reactions
MSP/E/050 A kit for 50 reactions
MSP/E/100 A kit for 100 reactions