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Nucleic Acid Extractions

Weak/missing signal for amplification of IC in HEX channel (HCV PCR Kit) when using manual extraction of RNA?

Possible causes 

  • Higher concentration of RNAses in clinical samples
  • Mistake during addition of IC
  • Incorrect storage conditions for IC

Problem solution

  • Add lysis buffer first, vortex with sample and then add IC
  • Control correct volume of IC which is calculated according elution volume (see IFU)
  • Check the storage conditions and expiration dates Internal Control (indicated on the tube).
Sample Real Time PCR

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Weak/missing signal for amplification of IC in HEX channel (HCV PCR Kit) when using croBEE NA extraction with a cartridge 201?

Possible causes

  • Higher concentration of RNAses in clinical samples
  • Error during the addition of IC
  • Incorrect storage conditions for IC

Problem solution

  • Add Proteinase K to a sample, vortex it and then incubate for 5 min. at RT. Continue with croBEE NA extraction.
  • Control the correct volume of IC which is calculated according to the elution volume (see IFU)
  • Check the storage conditions and expiration dates IC (indicated on the tube).
Sample Real Time PCR

Was the answer helpful?

YES NO Contact our technical support.

Weak/missing signal for amplification of IC in HEX channel when using manual extraction. The lysis buffer was mixed with Internal Control and stored in a refrigerator.

Possible causes

  • Instability of IC in lysis buffer
  • Problem with solubility of IC in thy lysis buffer

Problem solution

 

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Sample Real Time PCR

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YES NO Contact our technical support.

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