GeneProof Human Herpes Virus 8 (HHV-8) PCR Kit

CE IVD

PRODUCT DETAIL

DUAL TARGET DETECTION

  • Dual targeting prevents a detection failure caused by the occurrence of mutations

UNIVERSAL PCR PROFILE

  • Easily combinable with other PCR kits from the Immunocompromised / Transplanted group 
  • Simplifies laboratory workflow

QUALITATIVE AND QUANTITATIVE DETECTION

  • Unique possibility to quantify viral load

CONTAMINATION ELIMINATION

• Ready-to-Use Master Mix contains Uracil-DNA glycosylase (UNG) and dUTPs eliminating possible contamination with amplification products

 

TECHNICAL SPECIFICATION 

Technology real-time PCR
Type of Analysis qualitative and quantitative
Target Sequence

specific conservative DNA sequence of the ORF26 gene encoding the minor capsid protein (mCP)
specific conservative DNA sequence of the ORF73 gene encoding the latency-associated nuclear antigen (LANA)

Analytical Specificity

Human herpesvirus 8, 100%

Analytical Sensitivity (LoD)

reaches up to 2.225 cp/µl with the probability of 95%

Linear Range

109 – 10cp/ml with precision of ± 0.5 log

Reporting Units

cp/μl

Conversion Factor

not applicable

Metrological Traceability

not applicable

Extraction/Inhibition Control

PCR inhibition control (ISIN version)

PCR inhibition and DNA extraction efficiency control (ISEXversion)

Validated Specimen

cerebrospinal fluid (CSF), plasma, saliva, whole blood

Storage

-20 ± 5 °C

Validated Extraction Method

croBEE NA16 Nucleic Acid Extraction System

GeneProof PathogenFree DNA Isolation Kit

Instruments

croBEE Real-Time PCR System*

Applied Biosystems 7300 / 7500 Real-Time PCR System

CFX Connect™ / CFX96™ / Dx Real-Time PCR Detection System

LightCycler® 480

LineGene 9600 / 9600 Plus

Mic qPCR Cycler

QuantStudio™ 5 Real-Time PCR System

Rotor-Gene 3000 / 6000 / Q

SLAN® Real-Time PCR System
StepOne™ / StepOnePlus™ Real-Time PCR System

Required Detection Channels

FAM, HEX

External Quality Assessment

regularly tested by QCMD and Instand e.V. External Quality Assessment Panels - results at www.geneproof.com

Regulatory Status CE IVD 

* Validated Instruments

ORDER INFORMATION

Product name

Cat. No. Technology Package
GeneProof Human Herpes Virus 8 (HHV-8) PCR Kit HHV8/ISEX/025 real-time PCR 25 reactions
GeneProof Human Herpes Virus 8 (HHV-8) PCR Kit HHV8/ISEX/050 real-time PCR 50 reactions
GeneProof Human Herpes Virus 8 (HHV-8) PCR Kit HHV8/ISEX/100 real-time PCR 100 reactions