GeneProof Herpes Simplex Virus 1 (HSV-1) PCR Kit

CE IVD

PRODUCT ADVANTAGES

 

EXCELLENT EQA PANEL RESULTS

  • No penalty points for qualitative and quantitative detection since 2011 in 13 consequential EQA panels of INSTAND and QCMD

CONTAMINATION ELIMINATION

  • Ready-to-Use Master Mix contains Uracil-DNA glycosylase (UNG) and dUTPs eliminating possible contamination with amplification products

UNIVERSAL PCR PROFILE

  • Easily combinable with other PCR kits from the Immunocompromised / Transplanted, Neuroinfections and Sexually Transmitted Infections groups
  • Simplifies laboratory workflow

 

TECHNICAL SPECIFICATION 

Technology real-time PCR
Type of Analysis

qualitative and quantitative

Target Sequence

specific conservative DNA sequence of a single-copy gene encoding the glycoprotein B (gB)

Analytical Specificity

Herpes Simplex Virus type 1 (HSV-1), 100 %

Analytical Sensitivity (LoD)

reaches up to 122.124 cp/ml with the probability of 95 % (on AcroMetrixTM HSV-1 Plasma Panel using manual extraction GeneProof PahogenFree DNA Isolation Kit)

Linear Range 1010 – 102.5cp/ml with precision of ± 0.5 log

 

Reporting Units cp/μl

 

Conversion factor not applicable
Metrological Traceability

AcroMetrixTM HSV-1 Plasma Panel (AcroMetrix HSV-1)

Extraction/Inhibition Control

PCR inhibition control (ISIN version)
PCR inhibition and DNA extraction efficiency control (ISEX version)

Validated Specimen

whole blood, plasma, urine, CSF

Storage

-20 ± 5 °C

Validated Extraction Methods croBEE NA16 Nucleic Acid Extraction System
GeneProof PathogenFree DNA Isolation Kit
Instruments

croBEE Real-Time PCR System*
Applied Biosystems 7300 / 7500 Real-Time PCR System
AriaMx Real-Time PCR System
CFX Connect™ / CFX96™ / Dx Real-Time PCR Detection System
LightCycler® 2.0 / 480
LineGene 9600 / 9600 Plus
Mic qPCR Cycler
QuantStudio™ 3 / 5 Real-Time PCR System
Rotor-Gene 3000 / 6000 / Q
SLAN® Real-Time PCR System
StepOne™ / StepOnePlus™ Real-Time PCR System

Required Detection Channels FAM, HEX
External Quality Assessment regularly tested by QCMD and Instand e.V. External Quality Assessment Panels - for results see www.geneproof.com
Certification CE IVD 

*Validated Instruments

ORDER INFORMATION

Product name

Cat. No. Technology Package
GeneProof Herpes Simplex Virus 1 (HSV-1) PCR Kit HSV1/ISEX/025 real-time PCR 25 reactions
GeneProof Herpes Simplex Virus 1 (HSV-1) PCR Kit HSV1/ISEX/050 real-time PCR 50 reactions
GeneProof Herpes Simplex Virus 1 (HSV-1) PCR Kit HSV1/ISEX/100 real-time PCR 100 reactions