GeneProof Aspergillus PCR Kit

CE IVD

PRODUCT DETAIL

HIGH SENSITIVITY

  • Secured by targeting the multicopy sequence of the Intergenic spacer ITS2/28S rDNA

DIFFERENTIATION OF ASPERGILLUS TERREUS

  • Enables specific antifungal therapy of resistant aspergillosis

CONTAMINATION ELIMINATION

  • Ready-to-Use Master Mix contains Uracil-DNA glycosylase (UNG) and dUTPs eliminating possible contamination with amplification products

HIGH SPECIFICITY

  • Detection of a wide range of Aspergillus species
  • Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Aspergillus clavatus, Aspergillus nidulans, Aspergillus oryzae, Aspergillus ustus, Aspergillus versicolor, Aspergillus niveus, Aspergillus candidus, Aspergillus wentii, Aspergillus foetidus with differentiation of Aspergillus terreus

TECHNICAL SPECIFICATION 

Technology real-time PCR
Type of Analysis qualitative
Target Sequence Intergenic spacer ITS2/28S rDNA
Analytical Specificity

Aspergillus spp., 100 %
Aspergillus terreus, 100 %

Analytical Sensitivity (LoD)

48.837 genome/ml with 95 % probability (based on the analysis carried out in 2015)
31.43 copies/ml with the probability of 95 % (measured on Amplirun® Aspergillus fumigatus DNA control, Vircell, 2019)

Extraction/Inhibition Control

PCR inhibition and DNA extraction efficiency control (ISEX version)

Validated Specimen whole blood, plasma, BAL, sputum, CSF, serum
Storage -20 ± 5 °C
Validated Extraction Methods croBEE NA16 Nucleic Acid Extraction System
GeneProof PathogenFree DNA Isolation Kit
Instruments

croBEE Real-Time PCR System*
Applied Biosystems 7500 Real-Time PCR System
Aria Mx Real-Time PCR System
CFX96™/ Dx Real-Time PCR Detection System
LightCycler® 480
LineGene 9600/9600 Plus

Mic qPCR Cycler

QuantStudio™ 5 Real-Time PCR System
Rotor-Gene 3000/Q
SLAN® Real-Time PCR System

Required Detection Channels FAM, HEX, Cy5
External Quality Assessment regularly tested by QCMD External Quality Assessment panels – results at www.geneproof.com
Regulatory Status CE IVD

 * Validated instruments

 

ORDER INFORMATION

Product name

Cat. No. Technology Package
GeneProof Aspergillus PCR Kit ASP/ISEX/025 real-time PCR 25 reaction
GeneProof Aspergillus PCR Kit ASP/ISEX/050 real-time PCR 50 reaction
GeneProof Aspergillus PCR Kit ASP/ISEX/100 real-time PCR 100 reaction