GeneProof Flu Multiplex PCR Kit

The PCR kit is designed for Influenza A, Influenza B and RSV A/B detection by the real-time Polymerase Chain Reaction (PCR) method.

 

Product Advantages

 

MORE INFORMATION WITH LESS SAMPLES

  • Detects and distinguishes Influenza A, Influenza B and RSV in a single reaction tube
  • Suitable for differential diagnostics of infectious agents causing Flu and Flu-like symptoms

WHO RECOMMENDED DESIGN

  • Dual target detection of Influenza A (NP gene/M gene) and Influenza B

SINGLE TUBE READY-TO-USE MASTERMIX

  • Contains all components for PCR amplification
  • No additional PCR reagents pipetting necessary

HIGH SPECIFICITY

  • Detects all currently known Influenza A serotypes including H1N1, H1N1pdm09, H3N2, H5N8 and H7N9
  • Detects both Influenza B lineages Victoria and Yamagata
  • Detects RSV A and RSV B

CONTAMINATION ELIMINATION

  • Ready-to-Use Master Mix contains Uracil-DNA glycosylase (UNG) and dUTPs eliminating contamination with amplification products

 

TECHNICAL SPECIFICATION

 Technology real-time PCR
 Type of analysis qualitative
 Target Sequence specific region in M gene and NP gene for Influenza A virus
two specific regions in HA gene for Influenza B virus
specific region in M gene for Respiratory syncytial virus A and B
Analytical Specificity Influenza A virus, Influenza B virus and Respiratory syncytial virus A and B, 100 %
Analytical Sensitivity (LoD) reaches up to 25.75 cp/ml with the probability of 95 % for Influenza A virus (on INFA Medium Q Control, Qnostics, using manual extraction GeneProof PathogenFree RNA Isolation Kit)
reaches up to 161.64 cp/ml with the probability of 95 % for Influenza A virus (on INFA Medium Q Control, Qnostics, using automatic extraction croBEE NA16 Nucleic Acid Extraction System)
reaches up to 7.25 cp/μl with the probability of 95 % for Influenza B virus (on Amplirun® Influenza B RNA control, Vircell)
reaches up to 43.57 cp/μl with the probability of 95 % for Respiratory syncytial virus (on Amplirun® RSV A RNA control, Vircell)
Diagnostic Specificity 100.00% (CI95%: 79.95%–100.00%)
Diagnostic Sensitivity  92.31% (CI95%: 80.60%–97.51%)
Extraction/Inhibition Control PCR inhibition and RNA extraction efficiency control
 Validated Specimen

aspirate, BAL, swab, transport medium: Sigma MM*, Sigma Virocult*

 Storage -20 ± 5 °C
 Validated Extraction Methods croBEE NA16 Nucleic Acid Extraction System
GeneProof PathogenFree RNA Isolation Kit
 Instruments croBEE Real-Time PCR System*
Applied Biosystems 7300 / 7500 Real-Time PCR System
CFX Connect™ / CFX96™/ Dx Real-Time PCR Detection System
LineGene 9600 Plus
 Required Detection Channels FAM, HEX, Cy5, TexRed
 External Quality Assessment Regularly tested by QCMD and Instand e.V. External Quality Assessment Panels - results at www.geneproof.com
Regulatory Status

CE IVD

 

* Only in combination with GeneProof PathogenFreen RNA Isolation Kit

** Validated instruments

ORDER INFORMATION

Product name

Cat. No. Technology Package
GeneProof Flu Multiplex PCR Kit FLU/GP/025 real-time PCR 25 reactions
GeneProof Flu Multiplex PCR Kit FLU/GP/050 real-time PCR 50 reactions
GeneProof Flu Multiplex PCR Kit FLU/GP/100 real-time PCR 100 reactions