GeneProof Herpes Simplex Virus 2 (HSV-2) PCR Kit

CE IVD

PRODUCT ADVANTAGES

 

UNIVERSAL PCR PROFILE 

  • Easily combinable with other PCR kits from the Immunocompromised / Transplanted, Neuroinfections and Sexually Transmitted Infections groups 
  • Simplifies laboratory workflow

CONTAMINATION ELIMINATION

  • Ready-to-Use Master Mix contains Uracil-DNA glycosylase (UNG) and dUTPs eliminating possible contamination with amplification products 

QUALITATIVE AND QUANTITATIVE DETECTION 

  • Quantification of viral load provides additional information for management of HSV infection

 

TECHNICAL SPECIFICATION 

Technology real-time PCR
Type of Analysis qualitative and quantitative
Target Sequence specific conservative DNA sequence of a single-copy encoding the glycoprotein B (gB)

 

Specificity

Herpes simplex virus type 2 (HSV-2), 100 %

Sensitivity (LoD) reaches up to 197.215 cp/ml with the probability of 95 % (on AcroMetrixTM HSV-2 Plasma Panel using manual extraction GeneProof PathogenFree DNA Isolation Kit)

 

Linear Range

1010 - 102.5 cp/ml with precision of ± 0.5 log

Reporting Units

cp/μl

Conversion factor

not applicable

Metrological Traceability

AcroMetrixTM HSV-2 Plasma Panel (AcroMetrix HSV-2)

Extraction/Inhibition Control

 

PCR inhibition and DNA extraction efficiency control (ISEX version)

Validated Specimen

whole blood, plasma, urine, CSF

Storage

-20 ± 5 °C

Validated Extraction Method

croBEE NA16 Nucleic Acid Extraction System

GeneProof PathogenFree DNA Isolation Kit

Validated Instruments

croBEE Real-Time PCR System
Applied Biosystems 7300 / 7500 Real-Time PCR System
AriaMx Real-Time PCR System
CFX Connect™ / CFX96™ / Dx Real-Time PCR Detection System
LightCycler® 2.0 / 480
LineGene 9600 / 9600 Plus

Mic qPCR Cycler
QuantStudio™ 3 / 5 Real-Time PCR System
Rotor-Gene 3000 / 6000 / Q
SLAN® Real-Time PCR System
StepOne™ / StepOnePlus™ Real-Time PCR System

Required Detection Channels FAM, HEX
External Quality Assessment regularly tested by QCMD and Instand e.V. External Quality Assessment panels for results see www.geneproof.com
Certification CE IVD

 

ORDER INFORMATION

Product name

Cat. No. Technology Package
GeneProof Herpes Simplex Virus 2 (HSV-2) PCR Kit HSV2/ISEX/025 real-time PCR 25 reactions
GeneProof Herpes Simplex Virus 2 (HSV-2) PCR Kit HSV2/ISEX/050 real-time PCR 50 reactions
GeneProof Herpes Simplex Virus 2 (HSV-2) PCR Kit HSV2/ISEX/100 real-time PCR 100 reactions