GeneProof Factor II Prothrombin PCR Kit

CE IVD

thormbotic mutations

PRODUCT DETAIL

The PCR kit is designed to detect G20210A polymorphism of the gene for human factor II prothrombin by the real time Polymerase Chain Reaction (PCR) method.

The method consists in amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is the single nucleotide guanine/adenine polymorphism in site 20210 (G20210A). Presence of wild-type allele (G20210G) is detected in the FAM fluorescent channel and mutant allele (A20210A) in the HEX fluorescent channel.      In case of heterozygous genotype (G20210A) a signal is detected in both channels. Detection kit contains Ready to Use MasterMix and takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostics.

 

TECHNICAL SPECIFICATION

Technology real-time PCR
Type of Analysis qualitative
Target Sequence G20210A polymorphism of factor II prothrombin
Analytical Sensitivity reaches up to 2 ng/µl
Diagnostic Specificity 100 %
Diagnostic Sensitivity 100 %
Validated Specimen whole blood
Storage -20 ± 5 °C
Validated Extraction Methods croBEE NA16 Nucleic Acid Extraction System
GeneProof PathogenFree DNA Isolation Kit
Instruments

croBEE Real-Time PCR System*
Applied Biosystems 7300 / 7500 Real-Time PCR System
AriaMx Real-Time PCR System
CFX Connect™ / CFX96™ / Dx Real-Time PCR Detection System
LineGene 9600 Plus

Mic qPCR Cycler
QuantStudio™ 3 / 5 Real-Time PCR System
Rotor-Gene 3000
SLAN® Real-Time PCR System
StepOne™ / StepOnePlus™ Real-Time PCR System

Required Detection Channels FAM, HEX
External Quality Assessment regularly tested by Instand e.V. External Quality Assessment panels – results at www.geneproof.com
Regulatory Status CE IVD for in vitro Diagnostic Use

* Validated Instruments

ORDER INFORMATION

Product name

Cat. No. Technology Package
GeneProof Factor II Prothrombin PCR Kit FII/050 real-time PCR 50 reactions
GeneProof Factor II Prothrombin PCR Kit FII/100 real-time PCR 100 reactions
GeneProof Factor II Prothrombin PCR Kit FII/025 real-time PCR 25 reactions