GeneProof MTHFR A1298C PCR Kit

CE IVD

thrombotic mutations

 

PRODUCT DETAIL

The PCR kit is designed to detect A1298C polymorpism in the gene for methylenetetrahydrofolate reductase (MTHFR) by the real-time Polymerase Chain Reaction (PCR).

The method consists in amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is the single nucleotide adenine/cytosine polymorphism in site 1298 (A1298C). Presence of wild-type allele (A1298A) is detected in the FAM fluorescent channel and mutant allele (C1298C) in the HEX fluorescent channel. In case of heterozygous genotype (A1298C) a signal is detected in both channels. Detection kit contains Ready to Use MasterMix and takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostics.

 

TECHNICAL SPECIFICATION

Technology real-time PCR
Type of Analysis qualitative
Target Sequence A1298C mutation in the gene for methylenetetrahydrofolate reductase (MTHFR)
Analytical Sensitivity reaches up to 2 ng/µl
Diagnostic Specificity 100 %
Diagnostic Sensitivity 100 %
Validated Specimen whole blood
Storage -20 ± 5 °C
Validated Extraction Methods croBEE NA16 Nucleic Acid Extraction System
GeneProof PathogenFree DNA Isolation Kit
Validated Instruments

croBEE Real-Time PCR System
Applied Biosystems 7300 / 7500 Real-Time PCR System
AriaMx Real-Time PCR System
CFX Connect™ / CFX96™ / Dx Real-Time PCR Detection System
LightCycler® 2.0
LineGene 9600 / 9600 Plus

Mic qPCR Cycler
QuantStudio™ 3 / 5 Real-Time PCR System
Rotor-Gene 3000 / 6000
SLAN® Real-Time PCR System

Required Detection Channels FAM, HEX
External Quality Assessment Regularly tested by Instand e.V. External Quality Assessment panels – results at www.geneproof.com
Certification CE IVD for in vitro Diagnostic Use

ORDER INFORMATION

Product name

Cat. No. Technology Package
GeneProof MTHFR A1298C PCR Kit M1298/050 real-time PCR 50 reactions
GeneProof MTHFR A1298C PCR Kit M1298/100 real-time PCR 100 reactions
GeneProof MTHFR A1298C PCR Kit M1298/025 real-time PCR 25 reactions