GeneProof MTHFR C677T PCR Kit


thrombotic mutations


The PCR kit is designed to detect C677T polymorphism in the gene for methylenetetrahydrofolate reductase (MTHFR) by the real-time Polymeras Chain Reaction (PCR).

The method consists in amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is the single nucleotide cytosine/thymine polymorphism in site 677 (C677T). Presence of wild-type allele (C677C) is detected in the FAM fluorescent channel and mutant allele (T677T) in the HEX fluorescent channel. In case of heterozygous genotype (C677T) a signal is detected in both channels. Detection kit contains Ready to Use MasterMix and takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostics.



Technology real-time PCR
Type of Analysis qualitative
Target Sequence C677T mutation in the gene for methylenetetrahydrofolate reductase (MTHFR)
Analytical Sensitivity reaches up to 2 ng/µl
Diagnostic Specificity 100 %
Diagnostic Sensitivity 100 %
Validated Specimen whole blood 
Storage -20 ± 5 °C
Validated Extraction Methods croBEE NA16 Nucleic Acid Extraction System
GeneProof PathogenFree DNA Isolation Kit

croBEE Real-Time PCR System*
Applied Biosystems 7300 / 7500 Real-Time PCR System
AriaMx Real-Time PCR System
LightCycler® 2.0 / 480
LineGene 9600 Plus

Mic qPCR Cycler
QuantStudio™ 3 / 5 Real-Time PCR System
Rotor-Gene 3000 / 6000
SLAN® Real-Time PCR System
StepOne™ / StepOnePlus™ Real-Time PCR System

Required Detection Channels FAM, HEX
External Quality Assessment regularly tested by Instand e.V. External Quality Assessment panels – results at
Certification CE IVD for in vitro Diagnostic Use

* Validated Instruments


Product name

Cat. No. Technology Package
GeneProof MTHFR C677T PCR Kit M677/050 real-time PCR 50 reactions
GeneProof MTHFR C677T PCR Kit M677/100 real-time PCR 100 reactions