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The method consists in amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is the single nucleotide guanine/thymine polymorphism in site 103 (G103T). Presence of wild-type allele (G103G) is detected in the FAM fluorescent channel and mutant allele (T103T) in the HEX fluorescent channel. In case of heterozygous genotype (G103T) a signal is detected in both channels. Detection kit contains Ready-to-Use Master Mix and takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostics.
|Product name||Catalog no.||Technology||Packaging|
|GeneProof Factor XIII V34L PCR Kit||FXIII/025||real-time PCR||25 reactions|
|GeneProof Factor XIII V34L PCR Kit||FXIII/050||real-time PCR||50 reactions|
|GeneProof Factor XIII V34L PCR Kit||FXIII/100||real-time PCR||100 reactions|
|Type of Analysis||qualitative|
|Target Sequence||G103T Polymorphism of the F13A1 gene (V34L)|
|Diagnostic Specificity||100 %|
|Diagnostic Sensitivity||100 %|
|Validated Specimen||whole blood|
|Storage||-20 ± 5 °C|
|Validated Extraction Methods||croBEE NA16 Nucleic Acid Extraction System
GeneProof PathogenFree DNA Isolation Kit
croBEE Real-Time PCR System*
|Required Detection Channels||FAM, HEX|
|External Quality Assessment||regularly tested by Instand e.V. External Quality Assessment Panels - results at www.geneproof.com|
* Validated instruments
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