Welcome to GeneProof website!

It is great to see you here. To see all contents of the website, please register or restore your access.
We believe you will come back gladly.

GeneProof Team

 

User account
Forgot your password?
Searching
    • /
    • search

    Troubleshooting

    Troubleshooting

    System inside GeneProof

    Troubleshooting guide is divided according to the main themes. This division will allow for electronization and implementation into the GeneProof Web site, including a continuous update. Information sources for the updates are OTRS, discussion with customers and others. Troubleshooting data are updated repeatedly.

    The troubleshooting logic copy the diagnostics process in standard molecular diagnostic laboratories. The general topic is always written in capital letters, which is then further expand according to the specific problem.

    GENERAL – issue with low or no fluorescence

    Low fluorescence

    Possible Cause Solution
    Degraded MasterMix Store at -20 °C, thaw at 2 - 8 °C no longer than 30 min. Vortex and short spin before use.
    Unverified cycler Use verified cycler. List of verified cyclers here.
    Incorrect consumables Use recommended consumables. List of consumables in device manual.
    Clear PCR strips on BioRad CFX96 Use white PCR strips on BioRad CFX96
    Incorrect transport medium Use TE buffer or Nuclease-free water.
    Air bubbles in PCR mix solution Spin PCR strip before PCR.

    No fluorescence

    Possible cause Solution
    Incorrect PCR setup Contact support. Templates can be provided.
    Incorrect consumables Use recommended consumables. List of consumables in device manual.
    Incorrect transport medium Use TE buffer or Nuclease-free water.

     

    All

    Was the answer helpful?

    YES NO Contact our technical support.

    SAMPLE – issue with low fluorescence

    Possible Cause Solution
    Insufficient extraction Use validated extraction.
    Unvalidated specimen Check list of validated specimen in IFU.
    Mishandling with sample Check sampling and sample storage in IFU.
    All

    Was the answer helpful?

    YES NO Contact our technical support.

    SAMPLE – issue with non-sigmoidal curves

    Possible Cause Solution
    Invalid curves caused by software Contact support.
    All

    Was the answer helpful?

    YES NO Contact our technical support.

    SAMPLE – false positive sample

    Possible Cause Solution
    Contamination Clean workspace.
    Cross-talk Contact support.
    All

    Was the answer helpful?

    YES NO Contact our technical support.

    INTERNAL CONTROL – fail of internal control

    Possible Cause Solution
    Incorrect volume Add 10 % of elution volume to sample before extraction.
    Degraded IC Store at -20 °C, thaw at 2 - 8 °C no longer than 15 min.
    Insufficient extraction Use validated extraction.
    All

    Was the answer helpful?

    YES NO Contact our technical support.

    INTERNAL CONTROL – fail of internal control + positive sample

    Possible cause Solution
    Possible competition Normal - still valid result.
    All

    Was the answer helpful?

    YES NO Contact our technical support.

    INTERNAL CONTROL – fail of internal control + negative sample

    Possible cause Solution
    Failed extraction Use validated extraction. Repeat whole analysis.
    PCR inhibition Repeat analysis of failed samples.
    All

    Was the answer helpful?

    YES NO Contact our technical support.

    NEGATIVE CONTROL – positive signal

    Possible Cause Solution
    Contamination Clean workspace.
    All

    Was the answer helpful?

    YES NO Contact our technical support.

    POSITIVE CONTROL – negative signal

    Possible Cause Solution
    Degraded Positive Control Store at -20 °C, thaw at 2 - 8 °C no longer than 30 min. Vortex and short spin before use.
    Degraded MasterMix Store at -20 °C, thaw at 2 - 8 °C no longer than 30 min. Vortex and short spin before use.
    All

    Was the answer helpful?

    YES NO Contact our technical support.

    POSITIVE CONTROL – signal in HEX channel

    Possible Cause Solution
    Cross-talk Set threshold (TH) above curve in HEX.
    All

    Was the answer helpful?

    YES NO Contact our technical support.

    CALIBRATORS – issue with abnormal Ct

    Possible Cause Solution
    Incorrect pipetting Repeat PCR. Valid calibration curve values - R^2 ≥ 0,98; SLOPE between -3,8 to -3,1.
    All

    Was the answer helpful?

    YES NO Contact our technical support.

    SAMPLE – false negative sample

    Possible Cause Solution
    Incorrectly set threshold Set threshold lower/set threshold just above negative control
    All

    Was the answer helpful?

    YES NO Contact our technical support.

    Do you need more information?

    Contact Customer Support

    Contact form
    Choose file
    Choose file
    Choose file

    Your personal data will be processed according to the privacy policy .

    Order

    +420 543 211 679
    [obfuscate_1_|114|88|105|98|112|63|94|98|107|98|111|105|108|108|99|45|90|108|106]

    Customer Support 

    Send request
    +420 730 176 222
    [obfuscate_1_|114|108|109|109|108|113|107|61|100|98|109|92|109|111|108|110|93|43|96|108|108]

    FAQ

    News

    The biggest myths about IVDR

    Do you know the biggest myths that are connected with the new IVD Regulation?

    IVDR products available!

    All our Class „A“ products are now IVDR certified as required since May, 2022. Let’s see our offer!

    more