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The PCR kit is designed to detect C677T polymorphism in the gene for methylenetetrahydrofolate reductase (MTHFR) by the real-time Polymeras Chain Reaction (PCR).
The method consists in amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is the single nucleotide cytosine/thymine polymorphism in site 677 (C677T). Presence of wild-type allele (C677C) is detected in the FAM fluorescent channel and mutant allele (T677T) in the HEX fluorescent channel. In case of heterozygous genotype (C677T) a signal is detected in both channels. Detection kit contains Ready-to-Use Master Mix and takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostics.
|Product name||Catalog no.||Technology||Packaging|
|GeneProof MTHFR C677T PCR Kit||M677/025||real-time PCR||25 reactions|
|GeneProof MTHFR C677T PCR Kit||M677/100||real-time PCR||100 reactions|
|Type of Analysis||qualitative|
|Target Sequence||C677T mutation in the gene for methylenetetrahydrofolate reductase (MTHFR)|
|Diagnostic Specificity||100 %|
|Diagnostic Sensitivity||100 %|
|Validated Specimen||whole blood|
|Storage||-20 ± 5 °C|
|Validated Extraction Methods||croBEE NA16 Nucleic Acid Extraction System
GeneProof PathogenFree DNA Isolation Kit
croBEE Real-Time PCR System*
|Required Detection Channels||FAM, HEX|
|External Quality Assessment||regularly tested by Instand e.V. External Quality Assessment Panels - results at www.geneproof.com|
* Validated instruments
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