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GeneProof Team
The PCR kit is designed to detect C677T polymorphism in the gene for methylenetetrahydrofolate reductase (MTHFR) by the real-time Polymeras Chain Reaction (PCR).
The method consists in amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is the single nucleotide cytosine/thymine polymorphism in site 677 (C677T). Presence of wild-type allele (C677C) is detected in the FAM fluorescent channel and mutant allele (T677T) in the HEX fluorescent channel. In case of heterozygous genotype (C677T) a signal is detected in both channels. Detection kit contains Ready-to-Use Master Mix and takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostics.
Product name | Catalog no. | Technology | Packaging |
GeneProof MTHFR C677T PCR Kit | M677/025 | real-time PCR | 25 reactions |
GeneProof MTHFR C677T PCR Kit | M677/100 | real-time PCR | 100 reactions |
Technology | real-time PCR |
Type of Analysis | qualitative |
Target Sequence | C677T mutation in the gene for methylenetetrahydrofolate reductase (MTHFR) |
Diagnostic Specificity | 100 % |
Diagnostic Sensitivity | 100 % |
Validated Specimen | whole blood |
Storage | -20 ± 5 °C |
Validated Extraction Methods | croBEE NA16 Nucleic Acid Extraction System GeneProof PathogenFree DNA Isolation Kit |
Instruments |
croBEE Real-Time PCR System* |
Required Detection Channels | FAM, HEX |
External Quality Assessment | regularly tested by Instand e.V. External Quality Assessment Panels - results at www.geneproof.com |
Certification | CE IVD |
* Validated instruments
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support@geneproof.com
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