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The PCR kit is designed to detect G20210A polymorphism of the gene for human factor II prothrombin by the real time Polymerase Chain Reaction (PCR) method.
The method consists in amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is the single nucleotide guanine/adenine polymorphism in site 20210 (G20210A). Presence of wild-type allele (G20210G) is detected in the FAM fluorescent channel and mutant allele (A20210A) in the HEX fluorescent channel. In case of heterozygous genotype (G20210A) a signal is detected in both channels. Detection kit contains Ready-to-Use Master Mixand takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostics.
|Product name||Catalog no.||Technology||Packaging|
|GeneProof Factor II Prothrombin PCR Kit||FII/025||real-time PCR||25 reactions|
|GeneProof Factor II Prothrombin PCR Kit||FII/050||real-time PCR||50 reactions|
|GeneProof Factor II Prothrombin PCR Kit||FII/100||real-time PCR||100 reactions|
|Type of Analysis||qualitative|
|Target Sequence||G20210A polymorphism of factor II prothrombin|
|Diagnostic Specificity||100 %|
|Diagnostic Sensitivity||100 %|
|Validated Specimen||whole blood|
|Storage||-20 ± 5 °C|
|Validated Extraction Methods||croBEE NA16 Nucleic Acid Extraction System
GeneProof PathogenFree DNA Isolation Kit
croBEE Real-Time PCR System*
|Required Detection Channels||FAM, HEX|
|External Quality Assessment||regularly tested by Instand e.V. External Quality Assessment Panels - results at www.geneproof.com|
* Validated instruments
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