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GeneProof Team
The PCR kit is designed to detect G1691A polymorphism in the gene for human factor V Leiden by the real-time Polymerase Chain Reaction (PCR) method.
The method consists in amplification and detection of the target sequence using allele specific fluorophore labelled probes. The target sequence is a single nucleotide guanine/adenine polymorphism in site 1691 (G1691A). Presence of wild-type allele (G1691G) is detected in the FAM fluorescent channel and mutant allele (A1691A) in the HEX fluorescent channel. In case of heterozygous genotype (G1691A) a signal is detected in both channels. Detection kit contains Ready-to-Use Master Mix and takes advantage of the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Kit is designed for in vitro diagnostics.
Product name | Catalog no. | Technology | Packaging |
GeneProof Factor V Leiden PCR Kit | FV/025 | real-time PCR | 25 reactions |
GeneProof Factor V Leiden PCR Kit | FV/100 | real-time PCR | 100 reactions |
Technology | real-time PCR |
Type of Analysis | qualitative |
Target Sequence | G1691A polymorphism of factor V Leiden |
Diagnostic Specificity | 100 % |
Diagnostic Sensitivity | 100 % |
Validated Specimen | whole blood |
Storage | -20 ± 5 °C |
Validated Extraction Methods | croBEE NA16 Nucleic Acid Extraction System GeneProof PathogenFree DNA Isolation Kit |
Instruments |
croBEE Real-Time PCR System* |
Required Detection Channels | FAM, HEX |
External Quality Assessment | regularly tested by Instand e.V. External Quality Assessment Panels - results at www.geneproof.com |
Certification | CE IVD |
* Validated instruments
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support@geneproof.com
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